PHE_Scientific_ Analysis of iPSCs by G-banding

Descriptions

Public Health England (PHE) is seeking to procure an external service provider that can assess the chromosomal stability of human induced pluripotent stem cells (iPSCs). The proposed service must: -Be able to receive, handle and process live cells that are Advisory Committee on Dangerous Pathogens (ACDP) Hazard Group 2. -Have experience handling and fixing human induced pluripotent stem cells (iPSCs) e.g. identifying colony cultures that are in the log phase of growth. -Use Colcemid for arresting cells in metaphase so that chromosomes can be separated for cytogenetic studies. -Fix the cells using potassium chloride (KCl) solution and 3:1 methanol: acetic acid. -Assess the karyology of the fixed cells by G-banding - at least 20 G-banded metaphase spreads needed for each sample. -Provide a summary of the representative karyotype, along with additional information if any karyotypic abnormalities are found If you feel that you can meet the above requirements or would like more information, then please email Imran.rahman@phe.gov.uk.