Provision of imager/analyser for use in microtiter plate-based spot-counting bioassays

Descriptions

Public Health England (PHE) is seeking to procure a high-resolution imager/analyser for use in microtiter plate-based spot-counting bioassays. The proposed system must have: - specific capability of accurately counting viral plaques on mammalian cell monolayers in immunostained neutralisation assays, using both enzymatic and fluorescent detection - be able to accurately and reproducibly count the number of viral plaques per well - the capability of morphometric analysis allowing the measurement of plaque size and determining the percentage well area covered by plaques - the ability to accommodate 384 well plates - standard and high-resolution imaging capabilities for single and dual colour enzymatic analysis using visible light, ideally with automated colour compensation, and single, dual and triple colour fluorescence detection - the ability to perform high throughput cytotoxicity assays will also be an advantage - a software that allows for background artefacts (for example debris, hairs and fibres) to be eliminated from the count and have extensive quality control features - capability of assigning/printing bar codes for plate tracking and data management purposes is also an advantage. If you feel that you can meet the above requirements or would like more information, then please email imran.rahman@phe.gov.uk.