RQ0000010125 Epifluorescence Microscope

Descriptions

Intended uses (priority order): 1) Real-time imaging of cellular physiology using ion-sensitive fluorophores or similar probes (e.g. fura 2 and fluo dyes with ability for acquisition of fura 2 ratiometric images ≥1Hz) 2) General epifluorescence imaging of living cell cultures using a range of fluorescent dyes to image cellular structure and function (cell viability, mitochondrial function, ROS etc). Excitation/emission capabilities should cover standard range of fluorophores (e.g. DAPI, fluorescein, rhodamine etc). 3) General transmitted light and epifluorescence imaging of fixed / stained cell cultures and slide-mounted tissue sections Essential requirements: •Research-grade inverted microscope chassis, stage, transmitted light illumination, eyepieces etc •Microscope should be capable of phase and differential interference contrast transmitted light methods. •Objectives 10, 20, 40 and ~60 or 100x optimised for epifluorescence observation and DIC, 20 and 40x objectives optimised for UV excitation for Fura 2 (@340 & 380 nm, if different from standard epifluorescence objectives), additional 20 and 40x for phase contrast. •Epifluorescence light source to provide illumination for cellular physiology studies (excitation suitable for a range of dyes including fura 2) should include software controlled shutter, or equivalent, to control illumination and synchronise to image acquisition. •Epifluorescence light source for general epifluorescence imaging (2 and 3 above) if different •Ability to mount both epifluorescence illumination systems and switch between physiology and high-resolution epifluorescence imaging modes •Monochrome camera suitable for real-time imaging cellular physiology •Monochrome camera for high resolution general epifluorescence (if different from above) •Ability to mount both cameras and switch between physiology and high-resolution epifluorescence imaging modes •Image acquisition and analysis software suitable for running cellular physiology studies •Image acquisition and analysis software suitable for general epifluorescence studies. Ability to combine fluorescence images to produce composite images, ability to undertake image analysis/cytometry measurements of fluorescently labelled specimens. Desirable requirements: •Motorised control of microscope operation •Additional image analysis options for analysis of standard histopathology tissue sections Other: •If all capability is not possible on one microscope chassis then would consider two systems. This requirement will be on the defence sourcing portal (DSP)