LSO1387C - LSO1387C RFQ for the Provision of Proteomics Analysis Services for NUI Galway

Descriptions

To meet the research goals of CÚRAM and NUI Galway, we require to perform qualitative and quantitative Proteomics Analysis with experienced suppliers of this service. Proteomics is a large-scale comprehensive study of a specific proteome, including information on protein abundances, the variations and modifications, as well as their interacting partners and networks. Proteome analysis can be performed in two different strategies, bottom-up and top-down approaches, respectively. In the bottom-up approach, a crude protein mixture undergoes protease digestion first, and then separation by liquid chromatography, followed by Mass Spectrometry analysis. In the top-down method, proteins are characterized by Mass Spectrometry without prior proteolysis. Although bottom-up Proteomics is the most widely used method of proteome analysis provided by companies/facilities, top-down is also gaining prominence as it has the advantage of providing greater sequence coverage, resolution of sequence ambiguities and preservation of post translational modifications.